Difference between revisions of "Part:BBa K2984029"

 
 
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<partinfo>BBa_K2984029 short</partinfo>
 
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This part is composed of the PsaD promoter (BBa_K2984008), the GLE secretion signal (BBa_K2984001), the PETase enzyme (), a YFP mVenus marker (BBa_K2984020), the SP20 glycomodule for enhanced secretion (BBa_K2984024) and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984001">GLE</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984016">SP20</a> glycomodule for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.  
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===Usage and Biology===
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<partinfo>BBa_K2984029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984029 SequenceAndFeatures</partinfo>
  
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==Characterization==
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<figure>
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<img src="https://2019.igem.org/wiki/images/2/27/T--Humboldt_Berlin--Konstrukt_3.jpeg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 22:25, 19 October 2019


L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1314
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
    Illegal NgoMIV site found at 2466
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii