Difference between revisions of "Part:BBa K2984010"

Revision as of 22:19, 19 October 2019

L0 Backbone

This vector is a part of the Chlamy-HUB-Collection. Plasmid backbone for MoClo L0 cloning with an RFP insert for selection. L0-RFP-clone

Fig. 1 - Our self-constructed L0-RFP backbone transformed into E. coli (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2860
Illegal AgeI site found at 1014
Illegal AgeI site found at 1126
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 829
Illegal BsaI.rc site found at 1918

@@ Line 5: / Line 5: @@
 <html>
 This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Plasmid backbone for MoClo L0 cloning with an RFP insert for selection.
+<img src="https://2019.igem.org/wiki/images/3/3a/T--Humboldt_Berlin--notebook_s_1203.png" alt="L0-RFP-clone" width="500" />
+<figcaption>Fig. 1 - Our self-constructed L0-RFP backbone transformed into <i>E. coli</i> (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.</figcaption>
+</html>
@@ Line 19: / Line 24: @@
 <partinfo>BBa_K2984010 parameters</partinfo>
 <!-- -->
-<img src="https://2019.igem.org/wiki/images/3/3a/T--Humboldt_Berlin--notebook_s_1203.png" alt="L0-RFP-clone" width="500" />
-<figcaption>Fig. 1 - Our self-constructed L0-RFP backbone transformed into <i>E. coli</i> (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.</figcaption>
-</html>