Difference between revisions of "Part:BBa K2984005"
| (4 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| + | <partinfo>BBa_K2984005 short</partinfo> | ||
| + | <html> | ||
| + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes <i>BsaI</i> (GGAG/CGCT) and <i>BpiI</i> (GCAA/ACTA). | ||
| + | In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases. | ||
| + | </html> | ||
| + | |||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K2984005 SequenceAndFeatures</partinfo> | ||
Latest revision as of 22:10, 19 October 2019
Level 1 b backbone with GoldenGate Fusionsite
This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 729
Illegal XhoI site found at 721 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal AgeI site found at 1535
Illegal AgeI site found at 1647 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1823
Illegal BsaI.rc site found at 752