Difference between revisions of "Part:BBa K112105:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K112105 short</partinfo> | <partinfo>BBa_K112105 short</partinfo> | ||
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<pre> | <pre> | ||
| − | PCR | + | PCR mv008/mv007 on pBca9145-I716210 (667bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| − | Product is pBca1256- | + | Product is pBca1256-K112105 |
----------------------------------------------- | ----------------------------------------------- | ||
| − | + | mv008 Forward Biobricking of <r.BamHI> | |
| − | + | ccataGAATTCatgAGATCTgaagtagaaaaagagtttattactgatg | |
mv007 Reverse Biobricking of r.BamHI> | mv007 Reverse Biobricking of r.BamHI> | ||
cgttaGGATCCtttgttttcaactttatctttcc | cgttaGGATCCtttgttttcaactttatctttcc | ||
</pre> | </pre> | ||
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===Source=== | ===Source=== | ||
Revision as of 11:08, 21 October 2008
BamHI endonuclease without start or stop codons
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
PCR mv008/mv007 on pBca9145-I716210 (667bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112105 ----------------------------------------------- mv008 Forward Biobricking of <r.BamHI> ccataGAATTCatgAGATCTgaagtagaaaaagagtttattactgatg mv007 Reverse Biobricking of r.BamHI> cgttaGGATCCtttgttttcaactttatctttcc
Source
PCR mv005/mv007 on pBca9145-I716210 (670bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112104