Difference between revisions of "Part:BBa K2973013:Design"
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===References=== | ===References=== | ||
+ | Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059. | ||
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+ | Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002. | ||
+ | |||
+ | J. N. Zadeh, C. D. Steenberg, J. S. Bois, B. R. Wolfe, M. B. Pierce, A. R. Khan, R. M. Dirks, N. A. Pierce. NUPACK: analysis and design of nucleic acid systems. J Comput Chem, 32:170–173, 2011. | ||
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+ | http://www.nupack.org |
Latest revision as of 21:11, 19 October 2019
Trigger for Toehold switch 13 Geobacillus kaustophilus
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part was designed in order to achieve the transcription of a small part of the 16S rRNA gene of Geobacillus kaustophilus. This part behaves as a trigger sequence that will activate the Toehold switch 13 (BBa_K2973011).
Source
Geobacillus kaustophilus strain BGSC 90A1 16S ribosomal RNA, complete sequence
NCBI Reference Sequence: NR_115285.2
References
Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.
Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.
J. N. Zadeh, C. D. Steenberg, J. S. Bois, B. R. Wolfe, M. B. Pierce, A. R. Khan, R. M. Dirks, N. A. Pierce. NUPACK: analysis and design of nucleic acid systems. J Comput Chem, 32:170–173, 2011.
http://www.nupack.org