Difference between revisions of "Part:BBa K2984014"
 
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2<sup>+</sup> or Co2<sup>+</sup> making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.
 
This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2<sup>+</sup> or Co2<sup>+</sup> making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.
 
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This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
  
 
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<partinfo>BBa_K2984014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984014 SequenceAndFeatures</partinfo>
  
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==Characterization==
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<img src="https://static.igem.org/mediawiki/parts/2/27/T--Humboldt_Berlin--L0-6xHis-Tag_B5-B5.png" alt="Plate_L0-6xHis-Tag_B5-B5_E.coli" width="500">
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<figcaption>Fig.1 - Successful transformation of L0-6xHis-Tag_B5-B5 into <i>E. coli</i>.</figcaption>
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Latest revision as of 21:08, 19 October 2019


6x His-Tag for protein detection, isolation, purification B5-B5

This part is a part of the Chlamy-HUB-Collection. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2+ or Co2+ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Plate_L0-6xHis-Tag_B5-B5_E.coli
Fig.1 - Successful transformation of L0-6xHis-Tag_B5-B5 into E. coli.