Difference between revisions of "Part:BBa K2984012"
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This part was designed to be used with the MoClo standard and has B3-B4 overhangs. | This part was designed to be used with the MoClo standard and has B3-B4 overhangs. | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K2984012 SequenceAndFeatures</partinfo> | ||
==Characterization== | ==Characterization== | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 20:35, 19 October 2019
Hygromycin B CDS; Selection for Transformation - Antibiotic resistance aphVII (hyg) B3-B4
This part is a part of the Chlamy-HUB-Collection. Berthold et al. (2002)[1] found that the aminoglycoside phosphotransferase gene (aphVII) from Streptomyces hygroscopicus works as a selectable marker for C. reinhardtii establishing a hygromycin resistance.
This part was designed to be used with the MoClo standard and has B3-B4 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 797
Illegal NgoMIV site found at 905 - 1000COMPATIBLE WITH RFC[1000]
Characterization
