Difference between revisions of "Part:BBa K2984012"
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This part was designed to be used with the MoClo standard and has B3-B4 overhangs. | This part was designed to be used with the MoClo standard and has B3-B4 overhangs. | ||
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| + | ==Characterization== | ||
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| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/2/28/T--Humboldt_Berlin--L0-Hyg_B3-B4.png" alt="Plate_L0-Hyg_B3-B4_E.coli" width="500"> | ||
| + | <figcaption>Fig.1 - Successful transformation of L0-Hyg_B3-B4 into <i>E. coli</i>.</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
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Revision as of 20:33, 19 October 2019
Hygromycin B CDS; Selection for Transformation - Antibiotic resistance aphVII (hyg) B3-B4
This part is a part of the Chlamy-HUB-Collection. Berthold et al. (2002)[1] found that the aminoglycoside phosphotransferase gene (aphVII) from Streptomyces hygroscopicus works as a selectable marker for C. reinhardtii establishing a hygromycin resistance.
This part was designed to be used with the MoClo standard and has B3-B4 overhangs.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 797
Illegal NgoMIV site found at 905 - 1000COMPATIBLE WITH RFC[1000]