Difference between revisions of "Part:BBa K3179000:Design"

 
(→‎Source)
 
(4 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases  which can be devisable by three were added in order to separate ATGs.
+
This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts.
−
 
+
We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.
−
 
+
  
 
===Source===
 
===Source===
  
−
MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases  which can be devisable by three were added in order to separate ATGs.
+
We get this sequence from NCBI, and the sequence was synthesized from a biological company.
  
 
===References===
 
===References===
 +
1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.

Latest revision as of 18:23, 19 October 2019


miR663b-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts. We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.

Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References

1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.