Difference between revisions of "Part:BBa K2984066"

 
 
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. For the secretion of a protein in <i>Chlamydomonas reinhardtii</i> it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.
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For the secretion of a protein in <i>Chlamydomonas reinhardtii</i> it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K2984066 SequenceAndFeatures</partinfo>
 
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===MoClo Overhangs===
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This part with B2-B2 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">here</a>.
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<partinfo>BBa_K2984066 parameters</partinfo>
 
<partinfo>BBa_K2984066 parameters</partinfo>
 
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==References==
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Rasala, B. A., Lee, P. A., Shen, Z., Briggs, S. P., Mendez, M., & Mayfield, S. P. (2012). Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PloS one, 7(8), e43349.
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Latest revision as of 18:22, 19 October 2019


3'UTR, Secretion signal of Arylsulfatase ARS

For the secretion of a protein in Chlamydomonas reinhardtii it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B2-B2 MoClo overhangs can be found here.


References

  1. Rasala, B. A., Lee, P. A., Shen, Z., Briggs, S. P., Mendez, M., & Mayfield, S. P. (2012). Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PloS one, 7(8), e43349.