Difference between revisions of "Part:BBa K3187024"
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<partinfo>BBa_K3187024 short</partinfo>
 
<partinfo>BBa_K3187024 short</partinfo>
  
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The 6x His-Tag is used as a tag for detection and as an affinity tag in protein purification. An Imidazole gradient can later be used to elute the tagged protein from a column containing nickel.
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This part includes a C-terminal 6xHistidine-tag for the purification of heterologous expressed proteins and a upstream TAA Stop-codon.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:15, 19 October 2019


6x Histidine (His) Tag + TGA Stop Codon

This part includes a C-terminal 6xHistidine-tag for the purification of heterologous expressed proteins and a upstream TAA Stop-codon. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]