Difference between revisions of "Part:BBa K3179000:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts. | ||
We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell. | We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell. | ||
Revision as of 18:02, 19 October 2019
miR663b-target
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts. We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.
Source
This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts.
References
1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.