Difference between revisions of "Part:BBa K2969009"
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| − | An engineered CI transcription factor | + | An engineered CI transcription factor. We insert the TEV protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by TEV so that it will lose activity. |
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Latest revision as of 17:48, 19 October 2019
CI-TEVsite
An engineered CI transcription factor. We insert the TEV protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by TEV so that it will lose activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]