Difference between revisions of "Part:BBa K3081057"
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<partinfo>BBa_K3081059 short</partinfo> | <partinfo>BBa_K3081059 short</partinfo> | ||
| − | dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site | + | dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement of<partinfo>BBa_K3081040</partinfo>, which we add a degradation signal peptide ssrA to the dCas9. |
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Revision as of 17:48, 19 October 2019
dCas9 J23119 M+ with the thermo-sensitive transcription factor TCI42
dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement ofBBa_K3081040, which we add a degradation signal peptide ssrA to the dCas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 753
Illegal NheI site found at 776
Illegal NheI site found at 5286
Illegal NheI site found at 5309 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1297
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]