Difference between revisions of "Part:BBa K3311002"

 
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<partinfo>BBa_K3311002 short</partinfo>
 
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This composite part is a unit of “ HucR-pHucO-mCherry”(BBa_K3311004), which is responsible for mCherry (BBa_J06504) expressing.
  
 
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<partinfo>BBa_K3311002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3311002 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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The plasmid “pHucO-mCherry” was transformed to E. Coli DH5α for function test. As shown in Figure 1, The bacteria turned red after incubating overnight at 37 °C, which means the pHucO works well.
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[[File:PHucO.png|500px|thumb|left|Figure 1 Incubation of E. Coli containing the pSB1C3-pHucO-mCherry]]
  
 
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Revision as of 16:56, 19 October 2019


pHucO-mCherry

This composite part is a unit of “ HucR-pHucO-mCherry”(BBa_K3311004), which is responsible for mCherry (BBa_J06504) expressing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The plasmid “pHucO-mCherry” was transformed to E. Coli DH5α for function test. As shown in Figure 1, The bacteria turned red after incubating overnight at 37 °C, which means the pHucO works well.

Figure 1 Incubation of E. Coli containing the pSB1C3-pHucO-mCherry