Difference between revisions of "Part:BBa K3139001"
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This sequence can be added at the 5'-terminal of the RBS (between the promoter and RBS). In E.coli hosts, the ribosomal protein S1 could bind with the mRNA region which is encoded by this part, the stability of the mRNA and the possibility of ribosome binding shall rise at the same time. As a consequence, the expression of the gene which was added with this part can be obviously improved. | This sequence can be added at the 5'-terminal of the RBS (between the promoter and RBS). In E.coli hosts, the ribosomal protein S1 could bind with the mRNA region which is encoded by this part, the stability of the mRNA and the possibility of ribosome binding shall rise at the same time. As a consequence, the expression of the gene which was added with this part can be obviously improved. | ||
Expression of high-abundance proteins, like phage coat proteins, ATP synthase proteins, translational factors, or nucleoid proteins, is accompanied by an A/U-rich region upstream of the translation initiation codon and the ribosome-binding site, and this region acts as an "enhancer" by attracting ribosomal protein S1. | Expression of high-abundance proteins, like phage coat proteins, ATP synthase proteins, translational factors, or nucleoid proteins, is accompanied by an A/U-rich region upstream of the translation initiation codon and the ribosome-binding site, and this region acts as an "enhancer" by attracting ribosomal protein S1. | ||
| + | This part could be widely used as the effector gene - ribosomal protein S1 gene cannot get mutated as a lethal mutation. | ||
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Revision as of 16:13, 19 October 2019
A/U-rich 5-UTR
This sequence can be added at the 5'-terminal of the RBS (between the promoter and RBS). In E.coli hosts, the ribosomal protein S1 could bind with the mRNA region which is encoded by this part, the stability of the mRNA and the possibility of ribosome binding shall rise at the same time. As a consequence, the expression of the gene which was added with this part can be obviously improved. Expression of high-abundance proteins, like phage coat proteins, ATP synthase proteins, translational factors, or nucleoid proteins, is accompanied by an A/U-rich region upstream of the translation initiation codon and the ribosome-binding site, and this region acts as an "enhancer" by attracting ribosomal protein S1. This part could be widely used as the effector gene - ribosomal protein S1 gene cannot get mutated as a lethal mutation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]