Difference between revisions of "Part:BBa K3007000"
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It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid. | It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid. | ||
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==Contribution: WHU-China 2019== | ==Contribution: WHU-China 2019== | ||
'''Group:''' WHU-China | '''Group:''' WHU-China | ||
Revision as of 15:59, 19 October 2019
HucR binding site
It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid.
Contribution: WHU-China 2019
Group: WHU-China
Authors: Jiongyi He
Summary: We confirmed the function of sfGFP(BBa_I746916) and determined the relationship between sfGFP protein concentration and fluorescence intensity.
Documentation:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]