Difference between revisions of "Part:BBa K3171171"
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| − | V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is | + | V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive. |
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Revision as of 15:49, 19 October 2019
Vibrio natriegens native P1 promoter
V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 7
Illegal suffix found in sequence at 305 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal SpeI site found at 306
Illegal PstI site found at 320
Illegal NotI site found at 13
Illegal NotI site found at 313 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 7
Illegal suffix found in sequence at 306 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 7
Illegal XbaI site found at 22
Illegal SpeI site found at 306
Illegal PstI site found at 320 - 1000COMPATIBLE WITH RFC[1000]