Difference between revisions of "Part:BBa J70023:Design"

Latest revision as of 19:34, 20 October 2008

MF-plasmid-ori

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 548

Design Notes

No native restriction sites. Added biobrick ends.

The gene for PE contains no CGG codons.

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F

GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R

Alternate start; matches origin start in pSci1 plasmid

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2

Source

Genbank AJ969070

References

Breton M, Duret S, Arricau-Bouvery N, Béven L, Renaudin J. Characterizing the replication and stability regions of Spiroplasma citri plasmids identifies a novel replication protein and expands the genetic toolbox for plant-pathogenic spiroplasmas. Microbiology. 2008 Oct;154(Pt 10):3232-44. PMID: 18832328

Saillard C, Carle P, Duret-Nurbel S, Henri R, Killiny N, Carrère S, Gouzy J, Bové JM, Renaudin J, Foissac X. The abundant extrachromosomal DNA content of the Spiroplasma citri GII3-3X genome. BMC Genomics. 2008 Apr 28;9:195. PMID: 18442384

Berho N, Duret S, Danet JL, Renaudin J. Plasmid pSci6 from Spiroplasma citri GII-3 confers insect transmissibility to the non-transmissible strain S. citri 44. Microbiology. 2006 Sep;152(Pt 9):2703-16. PMID: 16946265

@@ Line 8: / Line 8: @@
 No native restriction sites.  Added biobrick ends.
+The gene for PE contains no CGG codons.
+GTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F
+GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R
+Alternate start; matches origin start in pSci1 plasmid
+GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2
 ===Source===