Difference between revisions of "Part:BBa J70023:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70023 short</partinfo>
 
<partinfo>BBa_J70023 short</partinfo>
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No native restriction sites.  Added biobrick ends.
 
No native restriction sites.  Added biobrick ends.
  
 +
The gene for PE contains no CGG codons.
  
 +
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F
 +
 +
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R
 +
 +
 +
Alternate start; matches origin start in pSci1 plasmid
 +
 +
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2
  
 
===Source===
 
===Source===
Line 16: Line 24:
  
 
===References===
 
===References===
 +
Breton M, Duret S, Arricau-Bouvery N, Béven L, Renaudin J.
 +
Characterizing the replication and stability regions of Spiroplasma citri plasmids identifies a novel replication protein and expands the genetic toolbox for plant-pathogenic spiroplasmas.
 +
Microbiology. 2008 Oct;154(Pt 10):3232-44.
 +
PMID: 18832328
 +
 +
Saillard C, Carle P, Duret-Nurbel S, Henri R, Killiny N, Carrère S, Gouzy J, Bové JM, Renaudin J, Foissac X.
 +
The abundant extrachromosomal DNA content of the Spiroplasma citri GII3-3X genome.
 +
BMC Genomics. 2008 Apr 28;9:195.
 +
PMID: 18442384
 +
 +
Berho N, Duret S, Danet JL, Renaudin J.
 +
Plasmid pSci6 from Spiroplasma citri GII-3 confers insect transmissibility to the non-transmissible strain S. citri 44.
 +
Microbiology. 2006 Sep;152(Pt 9):2703-16.
 +
PMID: 16946265

Latest revision as of 19:34, 20 October 2008

MF-plasmid-ori


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 548


Design Notes

No native restriction sites. Added biobrick ends.

The gene for PE contains no CGG codons.

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F

GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R


Alternate start; matches origin start in pSci1 plasmid

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2

Source

Genbank AJ969070

References

Breton M, Duret S, Arricau-Bouvery N, Béven L, Renaudin J. Characterizing the replication and stability regions of Spiroplasma citri plasmids identifies a novel replication protein and expands the genetic toolbox for plant-pathogenic spiroplasmas. Microbiology. 2008 Oct;154(Pt 10):3232-44. PMID: 18832328

Saillard C, Carle P, Duret-Nurbel S, Henri R, Killiny N, Carrère S, Gouzy J, Bové JM, Renaudin J, Foissac X. The abundant extrachromosomal DNA content of the Spiroplasma citri GII3-3X genome. BMC Genomics. 2008 Apr 28;9:195. PMID: 18442384

Berho N, Duret S, Danet JL, Renaudin J. Plasmid pSci6 from Spiroplasma citri GII-3 confers insect transmissibility to the non-transmissible strain S. citri 44. Microbiology. 2006 Sep;152(Pt 9):2703-16. PMID: 16946265