Difference between revisions of "Part:BBa K2996703:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2996600 short</partinfo>
+
<partinfo>BBa_K2996703 short</partinfo>
  
−
<partinfo>BBa_K2996600 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2996703 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
Cas 1 and 2 are flanked by 1 neucletide.
+
C-terminal is fused to dcas9 through a flexible linker.
  
  
  
 
===Source===
 
===Source===
−
 
+
This sequence is amplified from E. coli K12 genome.
−
This sequence is amplified from E. coli cas1-cas2 cassette, NEB 10-beta.  
+
  
 
===References===
 
===References===
 +
[1]. Bikard D, Jiang W, Samai P, etc. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Res, 2013, 41(15): 7429-37.
 +
[2]. Mathew R, Chatterji D. The evolving story of the omega subunit of bacterial RNA polymerase[J]. Trends in microbiology, 2006, 14(10): 450-5.
 +
[3]. Gourse R L, Ross W, Gaal T. UPs and downs in bacterial transcription initiation: The role of the alpha subunit of RNA polymerase in promoter recognition[J]. Molecular Microbiology, 2000, 37(4): 687-695.
 +
[4]. Murakami K S, Masuda S, Darst S A. Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution[J]. Science, 2002, 296(5571): 1280-1284.

Latest revision as of 15:17, 19 October 2019


RpoA, DNA-directed RNA polymerase subunit alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C-terminal is fused to dcas9 through a flexible linker.


Source

This sequence is amplified from E. coli K12 genome.

References

[1]. Bikard D, Jiang W, Samai P, etc. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Res, 2013, 41(15): 7429-37. [2]. Mathew R, Chatterji D. The evolving story of the omega subunit of bacterial RNA polymerase[J]. Trends in microbiology, 2006, 14(10): 450-5. [3]. Gourse R L, Ross W, Gaal T. UPs and downs in bacterial transcription initiation: The role of the alpha subunit of RNA polymerase in promoter recognition[J]. Molecular Microbiology, 2000, 37(4): 687-695. [4]. Murakami K S, Masuda S, Darst S A. Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution[J]. Science, 2002, 296(5571): 1280-1284.