Difference between revisions of "Part:BBa K3081115"
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<partinfo>BBa_K3081115 short</partinfo> | <partinfo>BBa_K3081115 short</partinfo> | ||
| − | + | The 20 bp sgRNA targets to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | |
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Revision as of 15:02, 19 October 2019
a 20bp sequence targeting the R3(top strand) DnaA box on E.coli oriC
The 20 bp sgRNA targets to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]