Difference between revisions of "Part:BBa K2943901"

 
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<partinfo>BBa_K2943901 short</partinfo>
 
<partinfo>BBa_K2943901 short</partinfo>
  
−
We've improved a previous RFP by using algorithms etc..
+
This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).<br>
 +
This sequence is based on the monomer RFP (mRFP, https://parts.igem.org/Part:BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were achieved by the program VienneRNA and optimize the mRNA folding energy to gain more efficient translation and expression.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:01, 19 October 2019


Improved mRFP

This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).
This sequence is based on the monomer RFP (mRFP, https://parts.igem.org/Part:BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were achieved by the program VienneRNA and optimize the mRNA folding energy to gain more efficient translation and expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]