Difference between revisions of "Part:BBa K2929001:Experience"
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===Applications of BBa_K2929001=== | ===Applications of BBa_K2929001=== | ||
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| + | ==iGEM 2019 St Andrews - Part Development== | ||
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| + | <p>The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.</p> | ||
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| + | <img src="https://2019.igem.org/wiki/images/2/2c/T--St_Andrews--Antibody_10_Expression_Test.jpg" width="30%"> | ||
| + | <p style="text-align:center;"><font size=-2>Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.</p> | ||
| + | </html> | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:59, 19 October 2019
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K2929001
iGEM 2019 St Andrews - Part Development
The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.
Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.
User Reviews
UNIQ872c160a270de8b2-partinfo-00000001-QINU UNIQ872c160a270de8b2-partinfo-00000002-QINU