Difference between revisions of "Part:BBa K2983025"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | [[File:T--Evry Paris-Saclay--Loop-Sites.png|300px|thumb| Figure 1. BsaI and SapI restriction sites (adapted from [1])]] The Loop Type IIS cloning sites (triangles in figures 1 and 2) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figures 1 and 2). | ||
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− | [[File:T--Evry Paris-Saclay--Loop Omega-F.png|300px|thumb|left| Figure | + | |
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+ | A total of 50 combinations are theoretically possible and this part is one of them: the loop Omega-F (Figure 2). | ||
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+ | [[File:T--Evry Paris-Saclay--Loop Omega-F.png|300px|thumb|left| Figure 2. Loop Omega-F structure]] | ||
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+ | This part was used to define the Loop assembly system dedicated to the oleaginous yeast ''Yarrowia lipolytica'' (for details visit https://2019.igem.org/Team:Evry_Paris-Saclay/Design) | ||
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+ | ===References=== | ||
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+ | [1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640. | ||
Revision as of 14:59, 19 October 2019
Loop Omega-F
This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in figures 1 and 2) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figures 1 and 2).
A total of 50 combinations are theoretically possible and this part is one of them: the loop Omega-F (Figure 2).
Blue triangle: SapI fixation site (GCTCTTC)
Red triangle : BsaI fixation site (GAGACC)
grey circle : overhang formed by SapI after cutting (GGT)
Yellow circle: overhang formed by BasI after cutting (CGCT)
This part was used to define the Loop assembly system dedicated to the oleaginous yeast Yarrowia lipolytica (for details visit https://2019.igem.org/Team:Evry_Paris-Saclay/Design)
References
[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 18
Illegal SapI site found at 1