Difference between revisions of "Part:BBa K2943900"
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<partinfo>BBa_K2943900 short</partinfo> | <partinfo>BBa_K2943900 short</partinfo> | ||
| − | This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902)that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).< | + | This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014). <br> |
| − | This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032)with several mutations inside and includes also base inserts that increase the Shine Dalgarno sequence length in order to optimize the expression of the mRFP in the composite part. | + | This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno(SD) sequence length in order to optimize the expression of the mRFP in the composite part. <br> |
| − | + | The mutations to optimize the SD were made according to the anti-SD sequence on the ribosome and the extra bases which were inserted were according to the the most common optimal distance in <em>E.coli.</em><br><br><br> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 14:52, 19 October 2019
Improved RBS
This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).
This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno(SD) sequence length in order to optimize the expression of the mRFP in the composite part.
The mutations to optimize the SD were made according to the anti-SD sequence on the ribosome and the extra bases which were inserted were according to the the most common optimal distance in E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]