Difference between revisions of "Part:BBa K3081107"
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The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved.  For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved.  For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  
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https://2019.igem.org/wiki/images/4/4d/T--Peking--different_DnaA_boxes_v4.png
  
  

Revision as of 14:46, 19 October 2019


a 20bp sequence targeting the M (bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058

T--Peking--different_DnaA_boxes_v4.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]