Difference between revisions of "Part:BBa K3196017"
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<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
This is a four section for degrade and transfer lignin part. | This is a four section for degrade and transfer lignin part. | ||
| − | [[File: | + | [[File:T--HUST--China--2019-FLO10-αpropelA.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αpropelA]] |
<h1>'''DNA Gel Electrophoretic'''</h1> | <h1>'''DNA Gel Electrophoretic'''</h1> | ||
Revision as of 14:45, 19 October 2019
AOX1-Kozak-FLO10 pro-pelA-His tag-AOX1 Terminator
FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. pelA can catalyze pectin.
Characterization
This is a four section for degrade and transfer lignin part.
DNA Gel Electrophoretic
After we link FLO10 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
SDS-PAGE
We run the SDS-PAGE to check whether FLO10 αpro help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
Enzyme Activity
We use DNS to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1986
Illegal BamHI site found at 937 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]