Difference between revisions of "Part:BBa K3081111"
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<partinfo>BBa_K3081111 short</partinfo> | <partinfo>BBa_K3081111 short</partinfo> | ||
| − | + | The 20 bp sgRNA targets to the R1- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | |
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Revision as of 14:44, 19 October 2019
a 20bp sequence targeting the R1(bottom strand) DnaA box on E.coli oriC
The 20 bp sgRNA targets to the R1- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]