Difference between revisions of "Part:BBa K3081105"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3081105 short</partinfo> | <partinfo>BBa_K3081105 short</partinfo> | ||
| − | A meaningless sequence. When it is flanked by J23119 promoter and sgRNA scaffold, the two BsaI site allows for replacement of this meaningless sequence into intended sgRNA sequence by Golden Gate assembly. It length (412bp) which is far longer than normal sgRNA (<20bp) can facilitate distinguishing successful Golden Gate product when the colony PCR fragment is loaded onto gel. | + | A meaningless sequence. When it is flanked by J23119 promoter and sgRNA scaffold, the two BsaI site allows for replacement of this meaningless sequence into intended sgRNA sequence by Golden Gate assembly. It length (412bp) which is far longer than normal sgRNA (<20bp) can facilitate distinguishing successful Golden Gate product when the colony PCR fragment is loaded onto gel.For more detailed information, see <partinfo>BBa_K3081058</partinfo> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:37, 19 October 2019
A meaningless sequence with two flanking BsaI sites,facilitating replacement of intended sgRNA
A meaningless sequence. When it is flanked by J23119 promoter and sgRNA scaffold, the two BsaI site allows for replacement of this meaningless sequence into intended sgRNA sequence by Golden Gate assembly. It length (412bp) which is far longer than normal sgRNA (<20bp) can facilitate distinguishing successful Golden Gate product when the colony PCR fragment is loaded onto gel.For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 216
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 406
Illegal BsaI.rc site found at 2