Difference between revisions of "Part:BBa K081014:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K081014===
 
===Applications of BBa_K081014===
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===Experiment 1 - performed by Lorenzo Pasotti and Mattia Quattrocelli===
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This generator was contained into pSB1AK3. The constitutive promoter BBa_J23100 was assembled upstream of the generator using BioBrick Standard Assembly.
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<br>
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After ligation reaction we transformed 1 ul of ligation product into 50 ul of Invitrogen TOP10 and plated transformed bacteria on LB agar plates with Ampicilline.
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<br>
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15 hours after plate incubation at 37°C, we put the plate on the transluminator. Positive transformants glowed under UV rays (see figure).
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<br>
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We also picked up some colonies from the plate using a tip and infected 1 ml of LB + Amp. After 3 hours of incubation (37°C, 220 rpm) we watched 50 ul of bacteria with TRITC channel. Results are reported in the following figure.
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<br>
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We can see that our RFP generator actually works.
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<br>
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{|align="center"
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|[[Image:pv_RED.jpg|600px]]
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|}
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{|align="center"
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|[[Image:pv_REDmicro.jpg|600px]]
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|}
  
 
===User Reviews===
 
===User Reviews===

Revision as of 20:27, 19 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K081014

Experiment 1 - performed by Lorenzo Pasotti and Mattia Quattrocelli

This generator was contained into pSB1AK3. The constitutive promoter BBa_J23100 was assembled upstream of the generator using BioBrick Standard Assembly.
After ligation reaction we transformed 1 ul of ligation product into 50 ul of Invitrogen TOP10 and plated transformed bacteria on LB agar plates with Ampicilline.
15 hours after plate incubation at 37°C, we put the plate on the transluminator. Positive transformants glowed under UV rays (see figure).
We also picked up some colonies from the plate using a tip and infected 1 ml of LB + Amp. After 3 hours of incubation (37°C, 220 rpm) we watched 50 ul of bacteria with TRITC channel. Results are reported in the following figure.
We can see that our RFP generator actually works.

Pv RED.jpg
Pv REDmicro.jpg

User Reviews

UNIQ3b8815bdc1cb980d-partinfo-00000000-QINU UNIQ3b8815bdc1cb980d-partinfo-00000001-QINU