Difference between revisions of "Part:BBa K3254026"
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This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ([[Part:BBa_K2615996|BBa_K2615996]]) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.  
 
This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ([[Part:BBa_K2615996|BBa_K2615996]]) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.  
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===Usage===
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[[File:T--GENAS_China--excision_with_backbone.PNG|200px|thumb|left| ]]
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We placed this part downstreamed a Ptac promoter([[Part:BBa_K2572025|BBa_K2572025]]) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see [[Part:BBa_K3254001|BBa_K3254001]]) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of Int5 integrase ([[Part:BBa_K3254006|BBa_K3254006]]).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:37, 19 October 2019


Translational Unit for Int5 integrase

This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Usage

T--GENAS China--excision with backbone.PNG

We placed this part downstreamed a Ptac promoter(BBa_K2572025) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see BBa_K3254001) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of Int5 integrase (BBa_K3254006).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
    Illegal AgeI site found at 118
    Illegal AgeI site found at 502
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]