Difference between revisions of "Part:BBa K2924043"
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The P<sub>HpaII</sub> promoter<sup>1</sup>, is a strong constitutive promoter that is commonly used for expression in gram-positive bacteria
 
The P<sub>HpaII</sub> promoter<sup>1</sup>, is a strong constitutive promoter that is commonly used for expression in gram-positive bacteria
 
===Usage and Biology===
 
===Usage and Biology===
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The promoter originated from the gram positive bacterium </i>Staphylococcus aureus</i> and has better affinity to the binding site of RNA polymerase or other transcription activators which help in increasing the transcription rate of downstream DNA to messenger RNA<sup>2,3,4</sup>. P<sub>HpaII</sub> promoter stimulates counterclockwise RNA synthesis<sup>5</sup>. Comparable expression strength to that of P<sub>43</sub> (BBa_K208002)<sup>2</sup>. In our case it was used to produce high amounts of heterologous protein (BBa_K2924033, BBa_K2924052) that is secreted into the culture supernatant.  
+
The promoter originated from the gram positive bacterium </i>Staphylococcus aureus</i> and has better affinity to the binding site of RNA polymerase or other transcription activators which help in increasing the transcription rate of downstream DNA to messenger RNA<sup>2,3,4</sup>. P<sub>HpaII</sub> promoter stimulates counterclockwise RNA synthesis<sup>5</sup>. Comparable expression strength to that of [[Part:BBa_K208002 P<sub>43</sub>]]<sup>2</sup>. In our case it was used to produce high amounts of heterologous protein (BBa_K2924033, BBa_K2924052) that is secreted into the culture supernatant.  
  
 
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Revision as of 13:05, 19 October 2019


Hpall promoter

The PHpaII promoter1, is a strong constitutive promoter that is commonly used for expression in gram-positive bacteria

Usage and Biology

The promoter originated from the gram positive bacterium </i>Staphylococcus aureus</i> and has better affinity to the binding site of RNA polymerase or other transcription activators which help in increasing the transcription rate of downstream DNA to messenger RNA2,3,4. PHpaII promoter stimulates counterclockwise RNA synthesis5. Comparable expression strength to that of [[Part:BBa_K208002 P43]]2. In our case it was used to produce high amounts of heterologous protein (BBa_K2924033, BBa_K2924052) that is secreted into the culture supernatant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: Westers, L., Dijkstra, D.S., Westers, H., van Diji, J.M., Quax, W.J. (2006). "Secretion of functional human interleukin-3 from Bacillus subtilis." Journal of biotechnology 123.2 (2006): 211-224.

[2]: Liu S. L., Du K. (2012). Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif. 2012 Jun;83(2):164-8. doi: 10.1016/j.pep.2012.03.015. Epub 2012 Apr 4

[3]: Guan C., Cui W., Cheng J., Zhou L., Guo J., Hu X., Xiao G., Zhou Z. (2015). Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015; 14: 150. doi: 10.1186/s12934-015-0341-2.

[4]: Chen, Jingqi, et al. "Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein." Biotechnology letters 37.4 (2015): 899-906.

[5]: Zhang, Kang, et al. "High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system." Microbial cell factories 16.1 (2017): 32.