Difference between revisions of "Part:BBa K3196019"

 
Line 3: Line 3:
 
<partinfo>BBa_K3196019 short</partinfo>
 
<partinfo>BBa_K3196019 short</partinfo>
  
−
protein
+
PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.
  
−
<!-- Add more about the biology of this part here
+
<h1>'''Characterization'''</h1>
−
===Usage and Biology===
+
This is a four section for degrade and transfer lignin part.
 +
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 +
 
 +
<h1>'''DNA Gel Electrophoretic'''</h1>
 +
After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
 +
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 +
 
 +
<h1>'''SDS-PAGE'''</h1>
 +
We run the SDS-PAGE to check whether FLO10 help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
 +
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 +
 
 +
<h1>'''Enzyme Activity'''</h1>
 +
We use DNS to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
 +
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
  
 
<!-- -->
 
<!-- -->

Revision as of 13:01, 19 October 2019


AOX1-Kozak-PHO1 pro-pelA-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. This figure shows the most possible kozak consensus sequence.

DNA Gel Electrophoretic

After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

SDS-PAGE

We run the SDS-PAGE to check whether FLO10 help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Enzyme Activity

We use DNS to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.

Figure1. This figure shows the most possible kozak consensus sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1968
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]