Difference between revisions of "Part:BBa K2973002"
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<b>Figure 1</b>: eGFP Fluorescence after <i>in vitro</i> protein expression.
 
<b>Figure 1</b>: eGFP Fluorescence after <i>in vitro</i> protein expression.
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:40, 19 October 2019


T7-RBS- enhanced green fluorescent protein (EGFP)

This composite part consists of T7 Promoter (BBa_J64997) and T7 Terminator(BBa_K731721), Ribosomal Binding Site(BBa_B0034) and EGFP CDS (BBa_K1094400). EGFP (Enhanced Green Fluorescent Protein) is a version of GFP(green fluorescent protein) that produces enhanced bioluminescence signal in the green zone of the noticeable spectrum and is being used widely as a protein reporter. This protein is produced by the jellyfish (Aequorea Victoria).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 776
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]