Difference between revisions of "Part:BBa K3015006"

 
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Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part [[Part:BBa_K145001|BBa_K14001]] to improve the applicability.
 
Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part [[Part:BBa_K145001|BBa_K14001]] to improve the applicability.
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The
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The T7-polymerase will start transcription from a T7-promotor<br>
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We Mutated the following basepairs to delete BbsI/BpiI recognition sites<br>
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1) S202 changed from TCT to TCC (bp 604-606)<br>
 +
2) E207 changed from GAA to GAG (bp 619-621)<br>
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3) E309 changed from GAA to GAG (bp 925-927)<br>
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4) E365 changed from GAA to GAG (bp 1093-1095)<br>
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5) K714 changed from AAG to AAA (bp 2140-21429<br>
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<br>
  
 
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Revision as of 12:32, 19 October 2019


T7 RNA Polymerase (No BbsI/BpiI)

Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part BBa_K14001 to improve the applicability. The

Usage and Biology

The T7-polymerase will start transcription from a T7-promotor
We Mutated the following basepairs to delete BbsI/BpiI recognition sites
1) S202 changed from TCT to TCC (bp 604-606)
2) E207 changed from GAA to GAG (bp 619-621)
3) E309 changed from GAA to GAG (bp 925-927)
4) E365 changed from GAA to GAG (bp 1093-1095)
5) K714 changed from AAG to AAA (bp 2140-21429

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]