Difference between revisions of "Part:BBa K3071013"

Revision as of 10:01, 19 October 2019

PspA promoter

Description

This is an regulatory element that originated from Escherichia coli K12 strain.

Biology

T--Hong Kong-CUHK--pspA promotor structure.png

Figure 1 Basic properties of pspA promotor

pspA promotor is a sigma-54 (σ-54) regulated activator dependent promotor. In the pspA promoter upstream region, it contains σ-54 consensus sequence 5' of the start contains a GG doublet as -24 and a consensus GG doublet at -12, the high-affinity IHF site (-25 to -60), as well as the UAS sites (UAS I: -89 to -107; UAS II:-111 to -129).

Usage

T--Hong Kong-CUHK--sigma 54 activation.png

Figure 2 The transcription initiation mechanism of the sigma-54 promotors [http://www.cchem.berkeley.edu/wemmer/research/sigma54.html]

sigm54-RNA holoenzyme (σ-54 RNAP) forms an inactive transcriptional initiation complex on this promotor, which can be activated in E. coli by the bacterial enhancer-binding protein PspF (BBa_K3071006). PspF functions by binding to the upstream activation sequences (UAS) near the promoter and contacting the promotor-bound σ-54 RNAP via DNA looping stabilized by the binding of integration host factor (IHF). Previous research has demonstrated the property of pspF-dependent and enhancer-specific transcription activation of pspA promotor.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 12
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3071013 short</partinfo>
+===Description===
+This is an regulatory element that originated from <i>Escherichia coli</i> K12 strain.
+===Biology===
+<center>[[File:T--Hong_Kong-CUHK--pspA_promotor_structure.png]]</center>
+<center><u> Figure 1 Basic properties of pspA promotor</u></center>
+pspA promotor is a sigma-54 (&#963;-54) regulated activator dependent promotor. In the pspA promoter upstream region, it contains &#963;-54 consensus sequence 5' of the start contains a GG doublet as -24 and a consensus GG doublet at -12, the high-affinity IHF site (-25 to -60), as well as the UAS sites (UAS I: -89 to -107; UAS II:-111 to -129).
-pspA promotor is a sigma-54 (&#963;-54) regulated activator dependent promotor. sigm54-RNA holoenzyme (&#963;-54 RNAP) forms an inactive transcriptional initiation complex on this promotor, which can be activated in E. coli by the bacterial enhancer-binding protein PspF (<html><a href="https://parts.igem.org/Part:BBa_K3071006">BBa_K3071006</a></html>). PspF functions by binding to the upstream activation sequences (UAS) near the promoter and contacting the promotor-bound &#963;-54 RNAP via DNA looping stabilized by the binding of integration host factor (IHF). Previous research has demonstrated the property of pspF-dependent and enhancer-specific transcription activation of pspA promotor.
+===Usage===
+<center>[[File:T--Hong_Kong-CUHK--sigma_54_activation.png]]</center>
+<center><u>Figure 2 The transcription initiation mechanism of the sigma-54 promotors [http://www.cchem.berkeley.edu/wemmer/research/sigma54.html]</u></center>
+<br>
+sigm54-RNA holoenzyme (&#963;-54 RNAP) forms an inactive transcriptional initiation complex on this promotor, which can be activated in E. coli by the bacterial enhancer-binding protein PspF (<html><a href="https://parts.igem.org/Part:BBa_K3071006">BBa_K3071006</a></html>). PspF functions by binding to the upstream activation sequences (UAS) near the promoter and contacting the promotor-bound &#963;-54 RNAP via DNA looping stabilized by the binding of integration host factor (IHF). Previous research has demonstrated the property of pspF-dependent and enhancer-specific transcription activation of pspA promotor.
+===Characterization===
 <!-- Add more about the biology of this part here