Difference between revisions of "Part:BBa K3221205"
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We expected amilCP to be expressed efficiently in <i>S. cerevisiae</i>, so we chose amilCP to be improved. Consequently, we processed codon optimization on amilCP and added a consensus sequence for its usage in <i>S. cerevisiae</i>. | We expected amilCP to be expressed efficiently in <i>S. cerevisiae</i>, so we chose amilCP to be improved. Consequently, we processed codon optimization on amilCP and added a consensus sequence for its usage in <i>S. cerevisiae</i>. | ||
We processed codon optimization and added a consensus sequence(AAAAAA) on the original amilCP sequence and obtained the sequence of yamilCP. | We processed codon optimization and added a consensus sequence(AAAAAA) on the original amilCP sequence and obtained the sequence of yamilCP. | ||
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Revision as of 09:23, 19 October 2019
YamilCP is a CDS of blue chromoprotein in S. cerevisiae.
YamilCP is a CDS of chromoprotein which can exhibit strong blue color in S. cerevisiae. It was optimized for S. cerevisiae on the basis of amilCP(BBa_K592009) which is encouraged by Team Uppsala-Sweden 2011. It acts as a reporter in our project to determine the function of Gal1 promoter (pGAL1) and Met3 promoter (pMet3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]