Difference between revisions of "Part:BBa K2951006"
 
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<partinfo>BBa_K2951006 short</partinfo>
 
<partinfo>BBa_K2951006 short</partinfo>
  
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LysRS fusion protein with polylinker
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__TOC__
  
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===Usage and Biology===
 
===Usage and Biology===
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This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI[https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3033657&to=3035174&strand=true (NC000913.3)]. LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.
  
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===Characterization===
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This part is part of the composite part: [https://parts.igem.org/Part:BBa_K2951008 BBa_K2951008] and further characterized.
  
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===Reference===
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===References===
 
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2444022/ 1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)]
 
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2444022/ 1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)]
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===<span class='h3bb'>Sequence and Features</span>===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2951006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2951006 SequenceAndFeatures</partinfo>
  

Latest revision as of 08:53, 19 October 2019

LysRS fusion protein

Usage and Biology

This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI(NC000913.3). LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.

Characterization

This part is part of the composite part: BBa_K2951008 and further characterized.

References

1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 275
    Illegal NotI site found at 1256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 864
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]