Difference between revisions of "Part:BBa K3165012:Design"

 
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===Design Notes===
 
===Design Notes===
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This part was designed by mutations at the 46th and 48th nucleotide bases, converting the start codon (ATG) to CTT, coding for Leucine. The part was codon optimised for Escherichia coli.
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This part was designed by mutations at the 46th and 48th nucleotide bases, converting the start codon (ATG) to CTT, coding for Leucine. The part was codon optimised for <i>Escherichia coli</i>.
  
  

Revision as of 07:15, 19 October 2019


i^2mCherry (Leu)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed by mutations at the 46th and 48th nucleotide bases, converting the start codon (ATG) to CTT, coding for Leucine. The part was codon optimised for Escherichia coli.


Source

The sequence was derived from the existing BioBrick (BBa_J18932 : coding sequence for mCherry).

References