Difference between revisions of "Part:BBa K2912005"

 
 
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SZU-China 2019 iGEM team was going to find a suicide switch inside the E coli that can break the whole body of the bacteria leading to the release of RNAi molecules transcribed from E coli inducing by IPTG or some other else. Therefore, we were in need the useful mechanism. Fortunately, we finally found the Refractile inclusion bodies (R-bodies) to kill the E coli, causing the inclusion to flow out of the plasma membrane, so that we can get the RNAi molecules transcribed by E coli.
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This is a single-stranded DNA that can be cyclized by the T7 promoter since it has two sites complementarity to the T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein CAP10A of M. micrantha（Unigene0004558）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.  
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Refractile inclusion bodies, known as R bodies, are produced by only a few species of bacteria. These inclusion bodies are highly insoluble protein ribbons, typically seen coiled into cylindrical structures within the cell[1]. R-bodies are produced by Paramecium endosymbionts belonging to the genus Caedibacter. These intracellular bacteria confer upon their hosts a phenomenon called the killer trait[2]. This is one of the DNA sequences for the R body locus (reb) from Caedibacter taeniospiralis.
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<center><html><img src='https://2019.igem.org/wiki/images/8/89/T--SZU-CHINA--fig._11%EF%BC%881%EF%BC%89.png' style="width:50%;margin:0 auto">
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<center> Fig.1 The Relative Target Gene Expression（Unigene0004558）after Using hairpin siRNA </center></html></center>
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The R bodies of C. taeniospiralis are type 51. They are about 0.5 &#956;m wide, have a maximum length of 20 &#956;m, and 13 nm thick, possess acute angles at each end, and unroll in a telescopic fashion when exposed to a pH of 6.5 or lower. These proteinaceous ribbons are rolling up inside the cell to form a hollow cylinder about 0.5 &#956;m in diameter and 0.5 &#956;m long.
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
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Latest revision as of 06:30, 19 October 2019


M. micrantha_leaves_ Unigene0004558

This is a single-stranded DNA that can be cyclized by the T7 promoter since it has two sites complementarity to the T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein CAP10A of M. micrantha（Unigene0004558）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.

Fig.1 The Relative Target Gene Expression（Unigene0004558）after Using hairpin siRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]