Difference between revisions of "Part:BBa K2912010"
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<partinfo>BBa_K2912010 short</partinfo> | <partinfo>BBa_K2912010 short</partinfo> | ||
| − | This is a single-stranded DNA that can be cyclized by T7 promoter | + | This is a single-stranded DNA that can be cyclized by T7 promoter since it has two sites complementary to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB7 of M. micrantha（Unigene0029127）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）. |
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| + | <center><html><img src='https://2019.igem.org/wiki/images/b/b7/T--SZU-CHINA--fig._1%EF%BC%886%EF%BC%89.png' style="width:50%;margin:0 auto"> | ||
| + | <center> Fig.1 The Relative Target Gene Expression after Using hairpin siRNA </center></html></center> | ||
| + | </div> | ||
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Revision as of 05:47, 19 October 2019
M. micrantha_leaves_ Unigene0029127
This is a single-stranded DNA that can be cyclized by T7 promoter since it has two sites complementary to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB7 of M. micrantha（Unigene0029127）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]