Difference between revisions of "Part:BBa K3105668"
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<partinfo>BBa_K3105668 short</partinfo> | <partinfo>BBa_K3105668 short</partinfo> | ||
| − | Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris. | + | Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>. |
HRP is often used as a conjugate with antibodies and detected by chemiluminescence. | HRP is often used as a conjugate with antibodies and detected by chemiluminescence. | ||
| − | The underlying reaction mechanism is described below: | + | The underlying reaction mechanism is described below: <br> |
HRP+ H2O2 = oxidased substrate | HRP+ H2O2 = oxidased substrate | ||
| + | |||
| + | <br> | ||
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Revision as of 00:04, 19 October 2019
Horseradish peroxidase
Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.
HRP is often used as a conjugate with antibodies and detected by chemiluminescence.
The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidased substrate
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 961
- 1000COMPATIBLE WITH RFC[1000]