Difference between revisions of "Part:BBa K3105667"

Revision as of 00:03, 19 October 2019

Aryl-alcohol oxidase

Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris

In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).

AAO facilitates following reaction:

primary aromatic alcohol + O2 = aromatic aldehyde + H2O2

References:
Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 150
Illegal BamHI site found at 337
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1297
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris
-In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide.
+In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).
 AAO facilitates following reaction:
 primary aromatic alcohol + O2 = aromatic aldehyde + H2O2
-<br>
+<br><br><br>
+<b>References:</b> <br>
+Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.
+<br><br>
 <!-- Add more about the biology of this part here