Difference between revisions of "Part:BBa K137114"

 
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<partinfo>BBa_K137114 short</partinfo>
 
<partinfo>BBa_K137114 short</partinfo>
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Inducible switch turning on rcsA, a regulator of capsule synthesis. Used to induce lambda phage lysogens to go into the lytic cycle.  
 
Inducible switch turning on rcsA, a regulator of capsule synthesis. Used to induce lambda phage lysogens to go into the lytic cycle.  
  
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===Usage and Biology===
 
===Usage and Biology===
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Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/rcsA_Lysogen_Induction rcsA lysogen induction protocol] and then [http://2008.igem.org/Team:Caltech/Protocols/Titering titering] the resulting phage on wild-type ''E. coli'', the series of devices S04080 (no promoter), K137129 (weak RBS B0033), K137118 (medium RBS B0032), K137114 (strong RBS B0034), and K137117 (strong RBS B0034 plus constitutive cI expression) produced the following concentrations of plaque forming units (pfu/mL):
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[[Image:RcsA.gif|700px]]
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Increasing the RBS strength increased the background lysogen induction without changing the
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K137114 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K137114 SequenceAndFeatures</partinfo>

Revision as of 18:09, 17 October 2008

AHL induced production of RcsA

Inducible switch turning on rcsA, a regulator of capsule synthesis. Used to induce lambda phage lysogens to go into the lytic cycle.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/rcsA_Lysogen_Induction rcsA lysogen induction protocol] and then [http://2008.igem.org/Team:Caltech/Protocols/Titering titering] the resulting phage on wild-type E. coli, the series of devices S04080 (no promoter), K137129 (weak RBS B0033), K137118 (medium RBS B0032), K137114 (strong RBS B0034), and K137117 (strong RBS B0034 plus constitutive cI expression) produced the following concentrations of plaque forming units (pfu/mL):

RcsA.gif

Increasing the RBS strength increased the background lysogen induction without changing the

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005