Difference between revisions of "Part:BBa K137076"
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Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Coculture_Inhibition_Assay coculture inhibition assay], a coculture of K137076 in pSB1A2 in JI377 was able to inhibit growth of JI377 containing pSB1AK3 (vs. no growth inhibition by cells containing an empty plasmid).
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[[Image:K137076.png]]
  
  

Revision as of 17:30, 17 October 2008

AHL inducible production of hydrogen peroxide

This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H2O2 production assay], K137076 in pSB1A2 in JI377 produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for JI377 with an empty plasmid).

K137076.gif

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Coculture_Inhibition_Assay coculture inhibition assay], a coculture of K137076 in pSB1A2 in JI377 was able to inhibit growth of JI377 containing pSB1AK3 (vs. no growth inhibition by cells containing an empty plasmid).

K137076.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005