Difference between revisions of "Part:BBa K137076"

 
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__NOTOC__
 
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<partinfo>BBa_K137076 short</partinfo>
 
<partinfo>BBa_K137076 short</partinfo>
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This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.
 
This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.
  
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===Usage and Biology===
 
===Usage and Biology===
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Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H<sub>2</sub>O<sub>2</sub> production assay], K137076 in pSB1A2 in DH10B produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for DH10B with an empty plasmid).
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[[Image:K137076.gif]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K137076 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K137076 SequenceAndFeatures</partinfo>

Revision as of 17:20, 17 October 2008

AHL inducible production of hydrogen peroxide

This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H2O2 production assay], K137076 in pSB1A2 in DH10B produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for DH10B with an empty plasmid).

K137076.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005