Difference between revisions of "Part:BBa K137018"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K137018 short</partinfo>
 
<partinfo>BBa_K137018 short</partinfo>
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This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.
 
This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.
  
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===Usage and Biology===
 
===Usage and Biology===
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When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], the response curve is shown below.
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[[Image:K137018.gif]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K137018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K137018 SequenceAndFeatures</partinfo>

Revision as of 17:06, 17 October 2008

AHL receiver + GFP

This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.


Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], the response curve is shown below. K137018.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1732