Difference between revisions of "Part:BBa K3110010"
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<partinfo>BBa_K3110010 short</partinfo> | <partinfo>BBa_K3110010 short</partinfo> | ||
| − | L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS. | + | This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS. |
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | ||
Revision as of 19:36, 18 October 2019
Weak Promoter Strong RBS lldD
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1202
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 639