Difference between revisions of "Part:BBa K2951007"

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===Short Description===
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===Usage and Biology===
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Hemagglutinin is is a homotrimericglycoprotein found on the surface of influenza viruses and integral to its infectivity.
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Hemagglutinin is a protein on the flu surface that plays a vital role in the virus’ capacity to infect cells, undergoing a conformational change when the virus binds to a host cell and becomes endocytosed. Influenza A viruses are divided into subtypes based on hemagglutinin (H) into 8 different hemagglutinin subtypes and can be used in immunological experiments as the target antigen.  
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This part contains the sequence of the hemagglutinin protein of Influenza A virus (A/WSN/1933 TS61(H1N1) obtained from NCBI GenBank(CY010788.1).
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This part contains the original sequence of Influenza A virus (A/WSN/1933 TS61(H1N1)) obtained from NCBI(CY010788.1) and a polylinker(Fig.1). It is originally from a previous part[https://parts.igem.org/Part:BBa_K1955000  BBa_K1955000]which differs in seven base pairs with the sequence from NCBI(CY010788.1). Hemagglutinin is one of the transmembrane proteins that are considered difficult to express as soluble form in E.coli. Thus this part has been codon optimized for E.Coli, and deleted its transmembrane domain analyzed by [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM Server v. 2.0](530-565, 36 codons) and the four extended hydrophobic amino acids in front of the domain.
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<br>
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<img src="https://2019.igem.org/wiki/images/3/3d/T--CSMU_Taiwan--improve1.png"
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style="width:200px; text-align: center;"><p>Fig.1 The polylinker sequence is shown above which contains several restriction enzyme cutting sites</p>
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===Characterization===
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This part is part of the composite part: [https://parts.igem.org/Part:BBa_K2951008 BBa_K2951008] and further characterized.
  
 
===References===
 
===References===
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Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64
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[https://www.sciencedirect.com/science/article/pii/S0166093413004497?via%3Dihub 1.Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64]
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(https://doi.org/10.1016/j.jviromet.2013.10.035)
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===<span class='h3bb'>Sequence and Features</span>===
 
===<span class='h3bb'>Sequence and Features</span>===

Latest revision as of 19:32, 18 October 2019

Influenza A hemagglutinin with poly linker

Usage and Biology

Hemagglutinin is a protein on the flu surface that plays a vital role in the virus’ capacity to infect cells, undergoing a conformational change when the virus binds to a host cell and becomes endocytosed. Influenza A viruses are divided into subtypes based on hemagglutinin (H) into 8 different hemagglutinin subtypes and can be used in immunological experiments as the target antigen. This part contains the original sequence of Influenza A virus (A/WSN/1933 TS61(H1N1)) obtained from NCBI(CY010788.1) and a polylinker(Fig.1). It is originally from a previous partBBa_K1955000which differs in seven base pairs with the sequence from NCBI(CY010788.1). Hemagglutinin is one of the transmembrane proteins that are considered difficult to express as soluble form in E.coli. Thus this part has been codon optimized for E.Coli, and deleted its transmembrane domain analyzed by [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM Server v. 2.0](530-565, 36 codons) and the four extended hydrophobic amino acids in front of the domain.

Fig.1 The polylinker sequence is shown above which contains several restriction enzyme cutting sites

Characterization

This part is part of the composite part: BBa_K2951008 and further characterized.

References

1.Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]